Behavioral Model Equipment

MBI Mouse Behavioral Core
Lead Investigator: Dr. Brooke Miller
Contact: Patricia Glenton, (352) 294-4970

Equipment

MedAssociates Open Field #MED-OFA (4 units)

• Open field arena consists of fixed position walls mounted to the system base.
• Movement is tracked through the use of three 16 beam IR arrays.
• IR beams are located on both the X and Y axes for positional tracking and Z axis for rearing detection.
• Test arena is compatible with ventilated cover, two chamber place preference insert, dark box insert, and hole board insert.
• Each test arena is enclosed in an environmental test chamber with lights and ventilating fan.
• Activity Monitor software.

MedAssociates Tail Suspension #MED-TSS (4 units)

• Tail Suspension 3-sided test cubicle.
• Removable tail hanger.
• Movement is measured by precision linear load cell with load cell amplifier and filter.
• Tail Suspension software.

MedAssociates T-maze with IR beam detection #MED-TMAZ-U (4 mazes)

• Three automatic guillotine doors allow for automated runway access control and eliminate the disruption caused by manual door manipulation.
• Dual sensors at the entrance to each goal runway allows software to differentiate between runway exploration and entrance resulting in more accurate position detection.
• Pellet receptacle and head entry detector at the end of each goal runway with 14 mg pellet dispensers allow for automated reinforcement.
• Interchangeable runways can be used with Y, Square, and 8 Arm Hubs allowing for added flexibility.
• T-maze Training and Testing software.

MedAssociates NIR Fear conditioning system #MED-VFC-SCT (2 units)

• Near-infrared video fear conditioning chamber with stainless-steel grid floor, quick disconnect harness, and house/stimulus light.
• Programmable tone generator, speaker, and sound calibration package.
• Aversive stimulator/scrambler and calibrator.
• Sound attenuating cubicle with exhaust fan and near infrared/visible lighting system.
• Visible light can be used within a session as a cue without impacting the video recording.
• Real time video recordings (30 frames per second) allows software to distinguish between subtle movements such as whisker twitch or tail flick and freezing behavior.
• Video Fear Conditioning Interface software.

San Diego Instruments Startle Response System #SR-LAB (4 units)

• Supports all startle paradigms, including acoustic startle response, startle habituation, pre-pulse and cross-model inhibition, fear-potentiated startle, trace conditioning and gap detection.
• Isolation cabinet with fan, light, and viewing lens.
• Complete sound generation system for white noise background and adjustable startle dB.
• Dynamic response sensor ignores static weight, allowing for full transducer range.
• Small/medium animal cylindrical enclosure and test base with sealed motion sensor.
• SR-LAB standardization unit for baseline calibration.
• SR-LAB software for test preparation, test execution, and data management.

TSE Systems Intellicage (2 cages)

• Fully automated screening for behavioral and cognitive functions of mice living in social groups in a home cage equipped with 4 programmable operant corners, up to 16 mice/cage.
• RFID transponder technology permits assessment of individual performance, stimulus delivery, and drug-treated water access.
• Uninterrupted short- or long-term monitoring, can be used under user-controlled light:dark conditions.
• 2 motorized doors block or allow access to water bottles on both sides of the corner.
• Air-puff valve for delivering negative reinforcement.
• Multicolor LEDs above the doors on both sides (conditional stimuli).
• Temperature-sensitive presence sensor detects the start, end, and duration of a corner visit.
• Nosepokes: interruption of a light-beam sensor at either door allows access to water bottles.
• Drinking: the number and duration of tongue-contacts with nipples of the bottles are registered by a lickometer.
• Programmable Designer, Controller, and Analyzer software package.
• Tasks can be programmed for the following behavioral domains:

1) Spontaneous behavior – anxiety, neophobia, exploration, behavioral stereotypes, habituation, circadian activity
2) Spatial and temporal behavior – place preferences and avoidance learning, reversal learning, spontaneous alternation, temporal conditioning, patrolling schedules
3) Discrimination learning – visual discrimination, gustatory discrimination, spontaneous drug preference or avoidance
4) Memory – habituation, working and reference memory, gustatory memory, procedural memory
5) Operant conditioning – fixed or progressive ratio conditioning, differential reinforcement of low responding (DRL)