Research Snapshot: Phillip Mackie and Habibeh Khoshbouei

By Michelle Jaffee

a colored microscope slide with a lot of green spots and a few pink spots inside the green.New research led by University of Florida neuroscientists shows that a protein called dopamine transporter, which is responsible for regulating levels of the chemical messenger dopamine in the brain, also might have a role in fine-tuning the response of specific immune cells in the blood to inflammation.

The study, published in the journal JCI Insight, applied live and fixed microscopy, super-resolution microscopy, electrophysiology, flow cytometry and biochemical techniques to human blood samples to study circulating immune cells.

Led by Habibeh Khoshbouei, Ph.D., Pharm.D., and Phillip Mackie, an M.D.-Ph.D. fellow in the Khoshbouei lab, the study builds upon their previous work investigating possible links between levels of dopamine — a neurotransmitter key to reward-seeking, motivation and movement — and inflammatory response in immune cells that are dysregulated in Parkinson’s disease.

The findings support a regulatory role for dopamine transporter in innate immune response and pave the way for further investigation into possible interaction with human immune cells called macrophages, Mackie said.

“Neurotransmitter transporters are thought to mainly exist in the cells of the brain where they control how neurons talk to one another,” he said. “However, we found that a subset of these proteins, namely the dopamine transporter and norepinephrine transporter, are also on peripheral immune cells called macrophages, one of the body’s essential defenders.”

The hope, Mackie said, is the findings will open a new path of research in the interdisciplinary field of neuroimmunology and prompt further study of neurotransmitter transporters and the peripheral immune system for both basic science and translational purposes.

“Our next step is to apply our findings to diseases of inflammation and neuroinflammation to ask how the dopamine transporter can be targeted in these conditions,” he said.

Read the paper in JCI Insight.