The MBI Cellular and Molecular Core provides researchers with advanced tools and expert guidance for single-cell, spatial and molecular biology workflows, spanning from sample preparation to data generation. Designed to support both emerging and experienced MBI investigators, the Core offers infrastructure to explore complex cellular and molecular mechanisms with precision and reproducibility.
Equipped with state-of-the-art 10x Genomics platforms for single-cell and spatial transcriptomics, the Core enables researchers to map cellular heterogeneity and tissue architecture at high resolution. Complementary instrumentation for nucleic acid analysis, imaging, protein quantification and sample preparation further supports a comprehensive range of molecular applications, empowering scientists to generate robust, high-quality data for impactful discoveries.
The core hub is located in L2-171 with some shared equipment and spaces available in other locations throughout the MBI.
Accessing the Core
The MBI Cellular and Molecular Core is available exclusively to MBI members. If you aren’t a member, you can become one here.
New users are encouraged to meet with our Core Directors before initiating single-cell or spatial workflows to ensure proper experimental setup and training.
Core Capabilities
Single-Cell and Spatial Transcriptomics
Capture cellular heterogeneity and tissue architecture with high resolution.
- 10X Genomics Chromium X Series – High-throughput single-cell RNA sequencing
- 10X Genomics Visium CytAssist – Spatial gene expression mapping
- ACD Hybridization Ovens – For RNAscope and in situ hybridization
Molecular Biology and Gene Expression
Support for molecular cloning, transfection, and quantitative gene expression.
- Agilent TapeStation, NanoDrop, Qubit, Countess 3 FL – RNA/DNA quantification and integrity checks
- VeritiPro Thermal Cycler and QuantStudio 3 qPCR System – Nucleic acid amplification and quantification
- Lonza 4D-Nucleofector – High-efficiency nucleic acid transfection
Protein, Imaging and Histology
Reliable imaging and quantification tools for protein and tissue research.
- Bio-Rad ChemiDoc MP and Li-Cor Odyssey FC – Multiplexed fluorescence and chemiluminescence imaging
- Leica CM1860 Cryostat – Precise tissue sectioning for histology
- MSD Platform and Microplate Reader – Protein expression and immunoassay analysis
Equipment Directory
| DESCRIPTION | MANUFACTURER | MODEL |
|---|---|---|
| Centrifuge 5810R | eppendorf | Centrifuge 5810R |
| Legend Micro 21R (MicroCentrifuge-Refrigerated) | Thermofisher | Legend Micro 21R |
| VeritiPro 96-Well Thermal Cycler | Thermofisher | VeritiPro 96-Well Thermal Cycler |
| QuantStudio 3 | Thermofisher | QuantStudio 3 |
| Nanodrop OneC | Thermofisher | Nanodrop OneC |
| Li-Cor | Li-Cor | Odyssey FC |
| Solaris 2000I (Orbital Shaker) | Thermofisher | Solaris 2000I |
| MESO QuickPlex SQ 120 | MSD | QuickPlex SQ 120 |
| gentleMACS Octo Dissociator | MACS Miltenyi Biotec | gentleMACS Octo Dissociator |
| TapeStation | Agilent Technologies | TapeStation |
All equipment in the table above is available in L2-171; however, MBI members also have access to shared equipment and spaces throughout the MBI.
Additional Shared Equipment
| Equipment/Facility | Room(s) |
|---|---|
| Darkrooms & X-ray Developer | L4-155 |
| Speedvac Rotary Evaporator | L4-155 |
| Autoclaves | L1-137, L2-137, L3-137, L4-137 |
| Perfusion Rooms | L1-139, L2-139, L4-139 |
| Cold Rooms | L1-141, L2-141, L3-141, L4-141 |
| Ice Machines Lab Use Only | LG-135, L1-130, L2-130, L3-130, L4-130 |
| Beckman LE-80K Ultracentrifuges | L2-158 |
| Beckman J2-HS Centrifuge | L2-158 |
| Sanyo Harrier 18/80 Refrigerated Centrifuge | L2-158 |
Contact Us
The Cellular and Molecular Core is a collaborative resource. Our experienced faculty provides consultation and ongoing support, guiding investigators from experimental design through data generation. The combination of cutting-edge instrumentation and expert guidance empowers researchers at all levels of experience to generate robust, high-quality data for impactful discoveries.
Eduardo Candelario-Jalil
Freddyson Martinez-Rivera
Additional Shared Equipment Manager
For questions regarding the additional shared equipment, please contact Dr. Ashok Kumar.
Ashok Kumar
Acknowledgment
Please include the following in your publications:
“Experiments were performed with support from the Evelyn F. and William L. McKnight Brain Institute, University of Florida.”
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